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The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
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The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
Quick Start Bradford Protein Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
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Boster Bio human mcp 1 picokinetm elisa kit
The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
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Boster Bio picokine human ptx 3 pre coated elisa kits
The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
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Human Protein Atlas quick-cfdna serum & plasma kit
The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
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Beijing Solarbio Science quick bca protein assay kit syw3-1
The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. <t>Quantified</t> <t>TNF-α</t> C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using <t>ELISA</t> assay. n = 3, ** p < 0.01, *** p < 0.001
Quick Bca Protein Assay Kit Syw3 1, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. Quantified TNF-α C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using ELISA assay. n = 3, ** p < 0.01, *** p < 0.001

Journal: Journal of Nanobiotechnology

Article Title: HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages

doi: 10.1186/s12951-021-01000-5

Figure Lengend Snippet: The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. Quantified TNF-α C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using ELISA assay. n = 3, ** p < 0.01, *** p < 0.001

Article Snippet: The supernatant was collected, centrifuged, and used for ELISA after 24, 48, and 72 h. According to the manufacturer’s protocol, TNF-α and IL-10 were quantified by ELISA Kit (FEK0527, EK0417, Boster).

Techniques: Fluorescence, Staining, Control, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay