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Image Search Results
Journal: Journal of Nanobiotechnology
Article Title: HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages
doi: 10.1186/s12951-021-01000-5
Figure Lengend Snippet: The polarization of macrophages to M2 phenotype is related to the elongated cell shape. A Fluorescence micrographs of Raw 264.7 macrophages immune-stained for arginase-1 (green), iNOS (red), and nuclear counterstain (blue) on cell plate (control), collagen and HA-collagen nanofibrous films. Scale bars: 15 μm. B Representative Western blot of arginase-1, iNOS, and tubulin of control, collagen and HA-collagen nanofibrous films and quantification of average across three separate experiments. Quantified TNF-α C and IL-10 D secretion from macrophages cultured on different nanofibrous scaffolds or culture plates using ELISA assay. n = 3, ** p < 0.01, *** p < 0.001
Article Snippet: The supernatant was collected, centrifuged, and used for ELISA after 24, 48, and 72 h. According to the manufacturer’s protocol, TNF-α and IL-10 were quantified by
Techniques: Fluorescence, Staining, Control, Western Blot, Cell Culture, Enzyme-linked Immunosorbent Assay